However, if no bubbles are observed, the culture is negative for catalase. The plate with the gram-negative is labeled and keep for future test. After observation, the H2S test had a negative result, which pointed to Escherichia coli. The milk agar was incubated at 37 degrees Celsius for 48 hours. You are encouraged to refer to this document each time you write a lab report. The gram positive bacteria did not turn yellow meaning acid was not produced, which verifies is the unknown gram positive bacteria. A lab report is a formal scientific document, and as such it differs from other forms of writing such as literature.
This article was co-authored by. A third test was done in order to confirm that Escherichia coli is the unknown. However, young children, the elderly, and those with a weak immune system are at great risk if they catch this strain. The Staphylococcus aureus and Enterococcus faecalis are positive to mannitol. Label the axes on a graph, being sure to include units of measurement. It is estimated that some 500,000 patients in American hospitals contract a staphylococcal infection each year. After observation there was a clear positive result, which showed the bacteria produced casease.
Kenyon College, 30 June 2006. After observation, a sample was taken from the isolated colony on the streak plate and another streak plate was done with that, trying to further isolate the colonies. Name: Danielle Title: Unknown Lab Report Introduction: There are many reasons for identifying an unknown bacterium. Identification of bacteria is important because they are not only useful but potentially dangerous as well. The Endospore stain is a special stain that is used to determine the presence of endospores in bacteria. This identification was reached by only one biochemical test and close observation.
In fact, the article will sound a bit similar to your report in that it will talk about objectives, materials and methods, and will have a conclusion and a discussion. The identification of bacteria can lead to breakthroughs in healthcare regarding treatment of old and new diseases alike. The unknown tube, 125, was isolated in order to grow two separate cultures. The conclusion section can also include lessons learned and future research areas that could shed further light on the current experiment. For example: the culture that I used it might be too old, or when I was inoculating the culture to the urea it might be hot, leaded to kill the bacterium. What are the 3 types of blood agar results and how can you recognize them? The broth starts out as red but if acid is produced the broth turns yellow.
A positive citrate test ruled out P. The following results were noted: Berb 1 — Isolated colonies — growth of one color Berb 2 — Isolated colonies — growth of one color Berb 3 was determined to be the best option distinctly different colonies. Table 1 lists the test, purpose, reagents and results. This shows that there are 5 possible answers: Bacillus cereus, Bacillus subtilis, Staphylococcus aureus, Staphylococcus epidermidis, and Enterococcus faecalis. The plate was placed in the incubator and the bacteria began to grow.
Second, people should know that pasteurized drinks are safer than unpasteurized. This is also where you would discuss any mistakes you might have made while conducting the investigation. Next an urea test was performed. Knowing what organism will give the student an idea of the genus and species that can cause diseases, how it can grow and reproduce including how this type of bacteria can be treated and killed, what type of antibiotic it will be susceptible to. It is important to know whether the unknown bacteria is Gram positive or Gram negative because Gram positive bacteria can be killed with antibiotics.
No clearing on the side of the streakNegativeGram - Organism does not produce gelatinase. No problems were encountered during the identification of this bacterium. In one sentence, state the hypothesis. The Gram stain revealed it was Gram negative rods. Also state if you have fulfilled the purpose of the experiment.
A nitrate broth was inoculated and incubated, afterwards reagent A and B were added to the solution and did not turn red so then zinc was added to the solution and the broth turned red meaning the nitrate did not reduce to nitrite. A healthy amount of E. Scientific writing is typically written in the passive voice. This results in complete clearing of the blood around the colonies. To avoid the spread and contamination of P. By going through the steps to identify the microorganism, students began to comprehend infectious disease.