Respirometer experiment with maggots. CPAC 9: Investigation into how temperature affects the rate of respiration using a respirometer 2018-12-23

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CPAC 9: Investigation into how temperature affects the rate of respiration using a respirometer

respirometer experiment with maggots

Carbon dioxide and water are products of the series of reactions involved in cellular respiration. I can now go into more detailed scientific theory of how the rate of respiration has increased. Therefore I will use a spatula when I need to remove some from the bottle. Evaluation We tried to keep our experiement under the same conditions by ensurong we used petri dishes so that the solutions didnt evaporate away. Yesterday the coordinator spoke of those in different communities often finding it hard to open up to their community about their disease particularly those living in Islamic or Hindu communities where the disease is less understood. This is respiration involving the consumption of oxygen gas, producing carbon dioxide and water, as shown below.

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Core practical 9: Investigate factors affecting the rate of aerobic respiration using a respirometer´╗┐

respirometer experiment with maggots

Oxygen is taken up by maggots for respiration, in one boiling tube, producing carbon dioxide, which is then absorbed by soda lime. Pyruvate is then transported into the mitochondrial matrix via active transport. Variables There are many variables that affect the rate or respiration, so I must take these into account when I am doing my experiments. A fly is a much more complex stage of the organism than the maggot, and so will respire faster. Aim: To investigate the effect of different light wavelengths on the photosynthesis of immobilised algae. There are two types of respiration that occur in cells to produce energy. The oxygen uptake is detected by.

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Core Practical 9: Investigating factors that affect the rate of aerobic respiration using a respirometer

respirometer experiment with maggots

We also didnt repeat our results which may have highlighted any anomalies in our data. This means the bonds holding the tertiary structure of the enzyme together will be overcome, and the active site will have altered in shape. Place 20 germinating seeds in the cylinder and record the volume by reading the new level of water. The enthalpy diagram below shows the overall change of glucose into carbon dioxide and water. Calculate the mean adjusted distance moved by the manometer fluid per minute. I will therefore have to work carefully and if I do burn myself I will immediately rinse my hand under cold water. Therefore, temperature is the dependent variable and light, noise, time and number of maggots are the independent variable.

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#96 Using respirometers

respirometer experiment with maggots

This will provide a safe working environment. There are several methods of indirectly measuring the rate of cellular respiration in organisms. Place the stoppers on each of the vials and ensure they are secured tightly. One vessel contains the organisms and the other acts as a thermobarometer. This would provide us more data points and give us a more accurate optimum temperature and points at which the enzyme denatures. This is illustrated in the diagram below.

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Effect of Temperature on the Rate of Reaction in Maggots Essay

respirometer experiment with maggots

Equally, he explained that the older generation were less inclined to join groups like the one he coordinated and was unsure of whether this was because of the stigma surrounding dementia, that is more understood in the younger generations, or whether it was something else like wanting to have the support of the group. Hydrolytic enzymes accelerate reactions in which a substance is broken down into simpler compounds through reaction with water molecules. Wear goggles and wash any splashes off the skin immediately. This function, known as aerobic respiration, is the reason mitochondria are frequently referred to as the powerhouse of the cell. Through the use of a catalase such as the potato, hydrogen peroxide is broken down to release water and oxygen. Crumb structure is shown to be important.

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Respiration maggots data logging experiment results oxygen sensor

respirometer experiment with maggots

Avoid castor-oil seeds as they are toxic if eaten. Submerge your respirometers, a little bit of water will enter the tips at first. If the gas inside the boiling tube was to heat up then this would cause pressure to increase inside the boiling tube. The reading shown on the above image would be. The air being drawn in from the outside to push the bubble along is at a lower pressure.

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Core practical 9: Investigate factors affecting the rate of aerobic respiration using a respirometer´╗┐

respirometer experiment with maggots

As there are no living organisms in the other boiling tube, the atmosphere does not change, but there is till soda lime present to remove any carbon dioxide. Close the tap to isolate the respirometer from the atmosphere and the syringe and start the stop clock immediately 6. Measure the distance travelled by the liquid during each minute the distance from one mark to the next on your pipette. I will now go into more detail about how the many enzymes involved in respiration are able to do this. To check that the apparatus is airtight, move the marker fluid in the manometer to one end with the syringe and leave for a few minutes. Two sets of respirometers will be assembled during this lab exercise. When assembling a respirometer, push the connections together firmly but gently to achieve an airtight joint with low risk of breaking any glass tubing.

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respirometer

respirometer experiment with maggots

Now that we have seen how respiration is able to release energy from glucose, we can consider the role enzymes play in the process. Good absorbent of Carbon Dioxide Tall 1L Beaker To contain. Therefore I will make sure that I cover the needle back up when I have finished using it. This allows the respirometer to measure only one variable, the consumption of oxygen gas by living cells. I must therefore bear this in mind when carrying out my investigation.

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CPAC 9: Investigation into how temperature affects the rate of respiration using a respirometer

respirometer experiment with maggots

After a few breaths the limewater in tube B will turn cloudy as the exhaled air contains more carbon dioxide. Ensuring the gauss fit tightly against the sides of the boiling tube can reduce this problem. Below is the table I constructed from the data gathered from the experiment, this particular experiment was recorded every 5 Celsius. Fill the graduated cylinder with 20ml of water. Alternatively fit a bung with a 3-way tap connected to the same items.

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