So why does butter and extra virgin olive oil make a lighter color together when one of them is extensively darker than the other, when in the lab the color of the tuna. Save this solution for analysis in step 4. We checked with spirit level that the glass plates were fixed on the suspension wall bracket were horizontal. In addition I have not given aid to another student on this assignment. If Benedict solution is added to gelatin, then it will turn. More iodine was added if the faint color disappeared.
These tests were done in test tubes. Each colony was carefully transferred to a nutrient plate agar, and then incubated at 37 C for 48 hours to promote growth of the pure culture. The reactants were measured out to the values calculated above in a graduated cylinder and then they were both poured in to the beaker. The tube was heated for 5 minutes at 60ºC if no precipitate formed. The identity of the unknown compound was determined by a flame and ion test to identify the cation and anion present in the compound. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics.
In this lab, 5 grams of an unknown white compound was discovered and its identity was narrowed down to a list of 15 compounds, all of which look similar. The bacteria can be found in soil, polluted water, raw meat, or in gastrointestinal tract of animals. After identification, the compound would have to be synthesized, in our case, through an acid-base reaction, followed by confirming its identity with the original unknown compound. The appropriate tests were prepared and applied. Identifying unknown chemical substances is an extremely important, and scientifically fantastic capability. We recorded the results in Table. If there is a visible clearing around bacteria growth indicating a positive result.
Some common household lipids are butter, canola oil, and extra virgin olive oil. The isolated Gram bacterium was taken on the inoculating loop and colored surface of the test tube. Introduction: During an inventory check of the chemistry stockroom, an unlabeled container was discovered and its contents were unknown. Materials and Methods:Streak isolation on a nutrient plate agar was performed to separate a mixture of bacteria to pure culture. Each one of these compounds has a different purpose.
Your compound may be a mixture and thus you must test for all possible cations and anions. The data collected were then used to calculate the volume, and later the density of the unknown metal. Also the butter was the lightest color of each the lipids and the extra virgin was the darkest. The fermentation tube contains broth with specific sugar, phenol red, and dye. The purpose of this lab is broken into two parts. The color, odor, melting point and boiling point determines the physical state and properties of a compound.
When bacterium produce nitrate reductase when grown in a medium containing nitrate, the enzymes will convert the nitrate to nitrite. Bacteria come in all shapes and sizes. The solubility test and the flame test were performed in the same way they were in day 1 and 2. You will work with a team to characterize the standards. It consists of a point mass suspended by a weightless, inextensible and flexible string from a fixed point about which the pendulum oscillates without friction.
The bacteria stain was Gram negative - with rod shape. Identification of an unknown compound is important to perform through the process of melting point M. Girls were told to wear ponytails. It consists of a heating. This process will alkalinize the agar and turn it blue. The Iodoform reaction would have a positive result if there is a pale yellow precipitate.
The Gram negative - bacteria possibilities were Escherichia coli, Klesiella Pneumoniae, Enterobacter aerogenes, Proteus Vulgaris, Pseudomonas aeruginosa. Mary Baniel Kristen Doucette David Swanson Tianlin Xu Unknown White Compound Purpose of the Lab What is the mysterious white compound? If Benedict solution is added to butter, then it will turn. It was left to stand for 3 minutes. Qualitative Procedure for the Analysis of Ca 2+ and Mg 2+ Ions 1. Without a favorable medium for growth Proteus vulgaris can survive 1-2 days. This indicates a positive test.